Antibiogram Studies of Salmonella Tshiongwe Isolates Obtained from a Broiler

| Salmonellosis is a common public health threat globally. Poultry have a major role in disseminating salmonellosis to humans. In this article, we report the presence of Salmonella Tshiongwe isolate from a broiler farm in western Uttar Pradesh, India and its antibiogram pattern. In the present study, 180 samples comprising of 160 poultry faeces, 5 feed and 15 drinking water samples were examined for the presence of Salmonella by growth enrichment on culture media. The suspected colonies obtained from selective media were further confirmed by biochemical tests, which consisted of Indole, Methyl Red, Voges Proskauer, Citrate, Triple Sugar Iron test, urease and motility tests. The presumptive Salmonella isolates obtained were serotyped at National Salmonella Centre, Indian Veterinary Research Institute, Bareilly, India according to Kauffmann-White scheme. The antibiotic susceptibility test of isolates was analyzed by Kirby-Bauer disc diffusion method. The biochemical testing of various samples collected from broiler farms showed a presence of 6.87% Salmonella in the samples, while serotyping revealed a prevalence of 1.88%. All the Salmonella isolates were obtained from faecal samples of broilers and none of the feed and water samples showed the presence of pathogen. The antimicrobial susceptibility test of isolates revealed that all the serotypes were resistant to Penicillin, Ampicillin, Gentamicin, Erythromycin, Sulpha-Trimethoprim, Tetracycline and Vancomycin. Most of the isolates were found to be highly sensitive to Amikacin, Azithromycin, Ceftriaxone, Ofloxacin, Ciprofloxacin and Chloramphenicol. The Salmonella isolates obtained were multidrug resistant. The presence of S. Tshiongwe in Indian broiler farms was reported for the first time and this serotype may be transmitted through the contaminated germplasm, spread by global trade.